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Home / Proteins & Peptide

Cas9, His-tag (S. pyogenes)

SKU : BHP18201108

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BPS Bioscience

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Mfr.No.	100206-3
Description	Streptococcus pyogenes CRISPR associated protein 9 (Cas9), also known as SpCas9, GenBank Accession No. NC_002737.2:854751-858857, a.a. 2-1368(end) with N-terminal HIs-tag, expressed in an E. coli cell expression system. MW = 160 kDa.
Immunogen Region	2-1368(end)
Tag	N-terminal His-tag
Formulation	20 mM HEPES, pH 7.5, 500 mM KCl, 20% Glycerol, 3 mM DTT.
Species	S. pyogenes
Application	Cas9 invitro cleavage assays to identify sgRNA with the highest cutting efficiency.
Notes	The Cas9 nuclease was incubated with sgRNA at 37°C for 10 minutes to form a functional RNP complex. A purified PCR product was then added at 37°C for 60 minutes, before Cas9 activity was visualized by agarose gel. Avoid freeze/thaw cycles.
GenBank	NC_002737.2:854751-858857
Mw(kda)	160 kDa

Synonyms	SpCas9
Uniprot	Q99ZW2

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Liang, X. et al., J Biotechnol. 2017; 241:136-146 2. Kim, S., et al., Genome Res. 2014; 24(6): 1012-1019

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201108	100206-3	500 ug	$715		Add to Cart

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