Human carbonic anhydrase II, also known as CA2, (GenBank Accession No. NM_000067), full length (a.a. 1-260(end)) with C-terminal His-tag, MW = 30 kDa, expressed in an E. coli cell expression system.
Specific activity: 1,400 pmol/min/ug
Immunogen Region
1-260(end)
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 106 mM imidazole and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
15 mM Tris-HCl, pH7.4, 100 mM NaCl, 3 mM p-Nitrophenylacetate (PNPA) and 0-2 ug CA2. Incubatefor 30 minutes at room temperature.Absorbance is measured at 405 nm.
Avoid freeze/thaw cycles.
GenBank
NM_000067
Mw(kda)
30 kDa
Synonyms
carbonic anhydrase II, CA2
Uniprot
P00918
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Loiselle, F.B., et al., Am J Physiol Cell Physiol. 2004 Jun;286(6):C1423-33 2. Shah, G.N., et al., Hum Mutat. 2004 Sep;24(3):272