Human BTK (GenBank Accession No. NM_000061), full length with N-terminal GST-tag, MW= 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 23 pmol/min/ug.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10 mM glutathione, 50% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate: 2 uM Tyr peptide 1 from Invitrogen.
Avoid freeze/thaw cycles.
GenBank
NM_000061
Mw(kda)
106 kDa
Synonyms
BTK, Bruton's tyrosine kinase
Uniprot
Q06187
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Liljeroos M., et al., Cell Signal. 2007 Mar,19(3):625-33. 2. Jou ST., et al., Int J Immunogenet. 2006 Oct,33(5):361-369