Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His-tag, MW=75 kDa, expressed in expressed in a baculovirus expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 412 pmoles/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (Ser/Thr 21, Invitrogen) at room temperature for 1 hour.