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Home / Proteins & Peptide

BRK, GST-tag

SKU : BHP18200234

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Mfr.No.	40403
Description	Human BRK, also known as PTK6 (GenBank Accession No. NM_005975), 2-451 (end), with N-terminal GST-tag, MW=80 kDa, expressed in Sf9 insect cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 88 pmol/min/ug
Background	BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain (1). BRK expression is low or undetectable in normal mammary tissue and benign lesions. However, approximately two-thirds of breast tumors express appreciable levels, and 27% of tumors over express BRK by fivefold or more (2).
Immunogen Region	2-451 (end)
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction was conducted in a buffer (25 ul) containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 uM ATP, 0.2 mg/ml poly-(Glu, Tyr), and BRK for 40 min at 30°C. ATP reduction is detected using ADP-Glo Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_005975
Mw(kda)	80 kDa

Synonyms	PTK6, FLJ42088
Uniprot	Q13882

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Mitchell, PJ. et al. Oncogene. 1994 Aug,9(8):2383-90. 2. Mitchell, PJ. et al. Oncogene. 1997 Sep 18,15(12):1497-502. Erratum in: Oncogene 1998 Jul 9,17(1):129.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200234	40403	10 ug	$390.50		Add to Cart

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