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Home / Proteins & Peptide

BRDT (BD1), His-tag

SKU : BHP18200074

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BPS Bioscience

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Mfr.No.	31101
Description	Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 22 - 138 corresponding to bromodomain 1 with N-terminal His-tag, MW = 14.9 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Immunogen Region	22-138
Tag	N-terminal His-tag
Formulation	50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	BRDT-BD1 (400 nM) is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) as indicated in BRD assay buffer (BPS #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA Ni2+ acceptor beads (Perkin Elmer) are added, followed by Alphascreen Streptavidin-conjugated donor beads (Perkin Elmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_207189
Mw(kda)	14.9 kDa

Synonyms	Bromodomain testis-specific protein, BRDT, BRD6, and CT9
Uniprot	Q58F21

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Berkovits, B.D., et al., Dev. Biol. 2011 Dec 15; 360(2): 358-368. 2. Shang, E., et al., Development. 2007 Oct; 134(19): 3507-3515.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200074	31101	100 ug	$335.50		Add to Cart

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