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Home / Proteins & Peptide

BRDT (BD1), GST-tag

SKU : BHP18200081

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Mfr.No.	31108
Description	Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 22 - 138 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Immunogen Region	22-138
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 8.0,138 mM NaCl, 2.7 mM KCl, 1 mM EDTA,10% glycerol and 1 mM DTT
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	BRDT protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA GSH acceptor beads (PerkinElmer) are added, followed by AlphaScreen Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_207189
Mw(kda)	40.7 kDa

Synonyms	BRDT, bromodomain testis-specific, BRDT, BRD6, and CT9
Uniprot	Q58F21

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Berkovits, B.D., et al., Dev. Biol. 2011 Dec 15; 360(2): 358-368 2. Shang, E., et al., Development. 2007 Oct; 134(19): 3507-3515

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200081	31108	100 ug	$335.50		Add to Cart

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