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Home / Proteins & Peptide

BRD4 (BD1+BD2), GST-tag

SKU : BHP18200065

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BPS Bioscience

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Mfr.No.	31044
Immunogen Region	49-460
Tag	N-terminal GST-tag
Description	Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 73.3 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Formulation	40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen Streptavidin -conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_058243
Mw(kda)	73.3 kDa

Synonyms	Bromodomain containing 4, BRD4, HUNK1, MCAP
Uniprot	O60885

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200065	31044	100 ug	$346.50		Add to Cart

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