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Home / Proteins & Peptide

BRD4 (49-460aa), His, Human

SKU : BHP18500343

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iXCells Biotechnologies

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Mfr.No.	IX03187, IX03187-100
Description	Bromodomain (BRD) is an extensive family of protein domains, originally identified in and named after the Drosophila protein Brahma. Members of BRD family share a conserved atypical left-handed four helix bundle structure, and specifically bind to epsilon-lysine acetylated proteins. It is well known that histone acetylation and methylation play a central role in epigenetics and are important for various gene transcription events, thus the acetyl-lysine binding property of BRDs make them suitable drug targets for epigenetics. Currently, there are 46 diverse human proteins containing 61 BRDs. These include histone acetyltransferases, ATP-dependent chromatin-remodeling complex proteins, and nuclear scaffold proteins. The main functions of BRDs in vivo include chromatin acetylation and deacetylation, nucleosome assembly and remodeling, and organizations of chromosome or chromatin domains.Recombinant human BRD4 (49-460) with His tag produced in E.coli is a single, non-glycosylated polypeptide chain containing 419 amino acids. A fully biologically active molecule, BRD4 (49-460) has a molecular mass of 47.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Formulation	Sterile liquid solution contains 25 mM HEPES, pH 7.5, 150 mM NaCl, 5% glycerol, 0.5 mM TCEP. Frozen solution.
Molecular Weight	47.5 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Accession Number	NM_058243
Endotoxin Level	1 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	Assay condition: 50 mM HEPES, 50 mM NaCl, and 1 mM CHAPS, pH 7.5. Biotinylated acetyl-histone peptide substrate binding to Bromodomain reaction was performed by adding 200 nM substrate to serially diluted BRD, and incubation for 1 h at RT. Treatment with equivalent detection buffer (2 nM Eu-Anti-His and 40 nM U-light-SA in Assay buffer), and incubation for 1 h at RT subsequently followed.

Synonyms	Bromodomain containing 4, HUNK1, MCAP.
Sequence	MHHHHHHETS NPNKPKRQTN QLQYLLRVVL KTLWKHQFAWPFQQPVDAVK LNLPDYYKII KTPMDMGTIK KRLENNYYWNAQECIQDFNT MFTNCYIYNK PGDDIVLMAE ALEKLFLQKINELPTEETEI MIVQAKGRGR GRKETGTAKP GVSTVPNTTQASTPPQTQTP QPNPPPVQAT PHPFPAVTPD LIVQTPVMTVVPPQPLQTPP PVPPQPQPPP APAPQPVQSH PPIIAATPQPVKTKKGVKRK ADTTTPTTID PIHEPPSLPP EPKTTKLGQRRESSRPVKPP KKDVPDSQQH PAPEKSSKVS EQLKCCSGILKEMFAKKHAA YAWPFYKPVD VEALGLHDYC DIIKHPMDMSTIKSKLEARE YRDAQEFGAD VRLMFSNCYK YNPPDHEVVAMARKLQDVFE MRFAKMPDE

Purity	95% by SDS-PAGE and HPLC analysis.
Storage	BRD4 (49-460) remains stable up to 6 months at -80°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing the cap. Aliquot to avoid repeated thawing and freezing.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500343-1	IX03187-100	100ug	$245		Add to Cart

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