Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 306 - 417 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 40 kDa, expressed in an E. coli expression system
host species: E. coli
Host species: E. coli
Immunogen Region
306-417
Tag
N-terminal GST-tag
Formulation
50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT
Species
Human
Application
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes
Enzyme was assayed according to BPS BRD3 (BD2) Assay Kit (Cat# 32523) protocol. BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen (R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.
Avoid freeze/thaw cycles.
GenBank
NM_007371
Mw(kda)
40 kDa
Synonyms
Bromodomain containing 3, BRD3, RING3L
Uniprot
Q15059
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640. 2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.