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Home / Proteins & Peptide

BRD3 (BD1), GST-tag

SKU : BHP18200057

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Mfr.No.	31032
Description	Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 - 145 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Immunogen Region	29-145
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	Enzyme was assayed according to BPS BRD3 (BD1) Assay Kit (Cat# 32513) protocol. BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen (R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_007371
Mw(kda)	40.7 kDa

Synonyms	Bromodomain containing 3, BRD3, RING3L
Uniprot	Q15059

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640. 2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200057	31032	100 ug	$335.50		Add to Cart

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