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Home / Proteins & Peptide

BRD3 (BD1+BD2), GST-tag

SKU : BHP18200060

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BPS Bioscience

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Mfr.No.	31035
Description	Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 - 417 corresponding to bromodomains 1 and 2, with N-terminal GST-tag, MW = 70 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Immunogen Region	29-471
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 30 min at RT. AlphaLISA GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen Streptavidin- conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_007371
Mw(kda)	70 kDa

Synonyms	Bromodomain containing 3, BRD3, RING3L
Uniprot	Q15059

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200060	31035	100 ug	$335.50		Add to Cart

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