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Home / Proteins & Peptide

BRD2 (BD2), GST-tag

SKU : BHP18200052

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BPS Bioscience

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Mfr.No.	31023
Description	Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 - 187 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 41.4 kDa, expressed in an E. coli expression system. host species: E. coli Host species: E. coli
Immunogen Region	339-459
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT
Species	Human
Application	Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes	Assay was performed using BRD2 BD2 Inhibitor Screening Assay Kit, Cat. #32522. BRD2 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 ul reaction for 1 hour at RT. AlphaLISA GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured. Avoid freeze/thaw cycles.
GenBank	NM_005104
Mw(kda)	41.4 kDa

Synonyms	BRD2, RING, RNF3, Bromodomain containing 2
Uniprot	P25440

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908. 2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200052	31023	100 ug	$335.50		Add to Cart

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