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Home / Proteins & Peptide

BRAF (V600E), GST, His-tags

SKU : BHP18200268

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BPS Bioscience

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Mfr.No.	40533
Description	Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), GenBank Accession No. NM_004333, a.a. 417-end with Val-to-Glu mutation on a.a. 600* and N-terminal GST-tag and His-tag, MW=69 kDa, expressed in Sf9 insect cells via a baculovirus expression system.*This mutation is often cited as V600E. Host cell line: Sf9 cells Specific activity: 150 pmol/min/ug
Background	BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of B-RAF (2).
Immunogen Region	417-end
Tag	N-terminal GST-tag, N-terminal His-tag
Formulation	40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in a kinase buffer containing 0.1 mg/ml inactive MEK1 substrate (BPS Cat# 40075) and 10 uM ATP. Reaction was done at 30°C for 30 minutes. ATP transferred was calculated using Kinase-Glo (Promega). Avoid freeze/thaw cycles.
GenBank	NM_004333
Mw(kda)	69 kDa

Synonyms	BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF (V600E)
Uniprot	P15056

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Weber, C.K., et al., Cancer Res. 2001 May 1, 61(9): 3595-8. 2. Mercer, K.E., et al., Biochim Biophys Acta. 2003 Jun 5, 653(1): 25-40.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200268	40533	10 ug	$390.50		Add to Cart

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