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Home / Proteins & Peptide

BRAF, GST-tag

SKU : BHP18200151

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BPS Bioscience

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Mfr.No.	40065
Description	Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 44 pmol/min/ug
Immunogen Region	417-766 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl₂, 0.1 mg/ml BSA)containing 2 ug inactive MEK1 (BPS #40075), 5 uM ATP, and 0.1 - 1.6 ng/ul BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents. Avoid freeze/thaw cycles.
GenBank	NM_004333
Mw(kda)	66 kDa

Synonyms	B-RAF1, BRAF, RAFB1
Uniprot	P15056

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780. 2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200151	40065	10 ug	$390.50		Add to Cart

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