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Home / Proteins & Peptide

BirA, His-FLAG-tags (E. coli-derived)

SKU : BHP18200554

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BPS Bioscience

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Mfr.No.	70030
Description	Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an E. coli cell expression system.
Immunogen Region	2-321 (end)
Tag	N-terminal His-FLAG-tags
Formulation	50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol.
Species	E. coli
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	BirA (10 ug/ml) completely biotinylated 10 uM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 uM d-Biotin. Avoid freeze/thaw cycles.
GenBank	NP_418404
Mw(kda)	37.1 kDa

Synonyms	Biotin protein ligase, biotin ligase
Uniprot	H0QFJ5

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7. 2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200554	70030	100 ug	$594		Add to Cart

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