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Home / Proteins & Peptide

BirA, GST-tag (E. coli derived)

SKU : BHP18200555

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BPS Bioscience

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Mfr.No.	70031
Description	Biotin ligase BirA, GenBank AccessionNo. NP_418404, a.a. 2 - 321 with NterminalGST-tag, MW = 61 kDa,expressed in an E. coli cell expressionsystem.
Immunogen Region	2-321 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol.
Species	E. coli
Application	Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Notes	BirA (0.16 mg/ml) completely biotinylated 10 uM of Avi-tag substrate at 30°C for 30 minutes in reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 uM d-Biotin. Avoid freeze/thaw cycles.
GenBank	NP_418404
Mw(kda)	61 kDa

Synonyms	Biotin protein ligase, biotin ligase
Uniprot	P06709

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Li, Y., et al., Protein Expr Purif. 2012 Mar, 82(1): 162-7. 2. Chakravartty, V., et al., J Bacteriol. 2012 Mar, 194(5): 1113-26.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200555	70031	100 ug	$566.50		Add to Cart

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