Recombinant human soluble basic FGF (GenBank Accession No. NM_002006) is a 17.3 kDa (134 aa-288 aa) protein expressed in an E. coli expression system.
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Immunogen Region
134 - 288
Tag
none
Formulation
Lyophilized from a 0.2 um filtered solution containing 5 mM Tris, pH 7.5, 150 mM NaCl
Species
Human
Application
Useful for cell culture and for the study of signaling pathways.
Endotoxin Level
< 1EU per ug of bFGF as determined by the LAL method.
Notes
Avoid freeze/thaw cycles.
Biological Activity Comment
Measured by its ability to stimulate proliferation of NIH3T3 cells. The ED50 of this effect is between 0.1 - 1 ng/ml
GenBank
NM_002006
Mw(kda)
17.3 kDa
Synonyms
Basic Fibroblast Growth Factor , FGF2, bFGF
Uniprot
P09038
Shipping Temperature
-20°C or -80°C
Format
lyophilized protein
Purity
>=98% by SDS-PAGE and HPLC analysis.
Storage
>=2 years (lyophilized) at -20°C.
Reference
1. Matsui, Y., et al. Cell 70(5), 841-847 (1992). 2. Segev, H, et al. Stem Cells. 22(3), 265-74 (2002).