PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
Immunogen Region
2091-2490
Tag
N-terminal GST-tag
Description
Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8kDa, expressed in an E. coli expression system.
Specific activity: 5 U/ug
Unit definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Formulation
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Species
Human
Application
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes
The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/ul enzyme for 5 minutes at 30°C.