Human secreted B7-H5 Fc fusion protein, also known as VISTA and VSIR, with C-terminal Avi-Tag. GenBank Accession No. NM_022153, a.a 33-194 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 47 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Immunogen Region
33-194
Tag
Fc Fusion, C-terminal Avi Tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_022153
Mw(kda)
47 kDa
Synonyms
VISTA, VSIR
Uniprot
Q9H7M9
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Zhu, Y., et al. Nature Communications. 2013 Feb 27; 4. 2. Jung, K., et al., Immune Network. 2013, Oct. 13;6:184-193.