Human secreted B7-H2, also known as B7 homolog 2, CD275, ICOSL, ICOSLG, and B7-related protein (B7RP), GenBank Accession No. NM_001283050, a.a 19-258, and expressed in a HEK293 cell expression system. MW = 31 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-258
Tag
C-Terminal Avi-His-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful as a positive control for Western blot studies.
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001283050
Mw(kda)
31 kDa
Synonyms
B7 Homolog 2, CD275, ICOSL, ICOSLG, and B7-Related Protein (B7RP)
Uniprot
O75144
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution.
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Guillonneau, C., et al., Transplantation. 2005; 80(4): 546-554.2. Fan, X., et al., J. Exp. Med. 2014; 211(4): 715-725.