Human secreted B7-H2, also known as B7 homolog 2, CD275, ICOSL, ICOSLG, and B7-related protein (B7RP), GenBank Accession No. NM_001283050, a.a 19-258, with C-terminal Avi-His-Tag and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag technology. MW = 31 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-258
Tag
Avi-His-Tag, Biotin-Labeled
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for studying protein binding and screening small molecular inhibitors
GenBank
NM_001283050
Mw(kda)
31 kDa
Synonyms
7 Homolog 2, CD275, ICOSL, ICOSLG, and B7-Related Protein (B7RP), Biotin
Uniprot
O75144
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Guillonneau, C., et al., Transplantation. 2005; 80(4): 546-554. 2. Fan, X., et al., J. Exp. Med. 2014; 211(4): 715-725.