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Home / Proteins & Peptide

B7-1(CD80) (Mouse), Fc fusion, Avi-Tag, Biotin-labeled, HiP

SKU : BHP18201175

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BPS Bioscience

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Mfr.No.	100478-2
Description	Mouse secreted B7-1, also known as CD80, GenBank Accession No. NM_009855, a.a 38-246, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag technology. MW=52 kDa. Biotinylation is confirmed to be>=90%.
Immunogen Region	38-246
Tag	C-terminal Fc-Avi-tag
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species	Mouse
Application	Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes	Reaction done similar to CTLA4:B7-1(biotin) assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (mouse CTLA4) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (mouse B7-1(B)) (0-50 ng) added for 2h at RT. Strep-HRP added for 1h at RT. HiP indicates a high purity protein with>=90% purity as measured by gel filtration and aggregation<10%. Avoid freeze/thaw cycles.
GenBank	NM_009855
Mw(kda)	52 kDa

Synonyms	CTLA-4 Counter-Receptor B7.1, CD80
Uniprot	Q00609

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Pentcheva-Hoang, T., et. al. Immunity. 2004 Sep; 21(3): 401-413. 2. Hirano, F., et al., Cancer Research. 2005 Feb; 65(3): 1089-1096.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201175	100478-2	50 ug	$478.50		Add to Cart

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