Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Immunogen Region
35-242
Tag
C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_005191
Mw(kda)
52.5 kDa
Synonyms
CTLA-4 Counter-Receptor B7.1
Uniprot
P33681
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.