AXL (GenBank Accession No. NM_021913), a.a. 473-894 with N-terminal GST-tag, MW= 77 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 140 U/mg.
Unit definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
Immunogen Region
473-894
Tag
N-terminal GST-tag
Formulation
5 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant AXL.
Avoid freeze/thaw cycles.
GenBank
NM_021913
Mw(kda)
77 kDa
Synonyms
AXL, UFO, JTK11
Uniprot
P30530
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Sawabu T. et al., Mol Carcinog. 2007 Feb,46(2):155-64. 2. Vajkoczy P. et al., Proc Natl Acad Sci U S A. 2006 Apr 11,103(15):5799-804.