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Home / Proteins & Peptide

Aurora Kinase B, GST-tag, His-tag

SKU : BHP18200123

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BPS Bioscience

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Mfr.No.	40002
Description	Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 6.2 U/ug. Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.
Immunogen Region	2-344
Tag	N-terminal GST-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay buffer: 50 mM HEPES,pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mMDTT, 3 uM Na-orthovanadate, 0.5 mMATP, 4 uM aurora B substrate, and 4ug/ml Aurora B kinase. Avoid freeze/thaw cycles.
GenBank	NM_004217
Mw(kda)	66 kDa

Synonyms	Aurora Kinase B, STK12, STK5, AIM-1, IPL1, ARK2, AURB
Uniprot	Q96GD4

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Delaval, B. et al., Oncogene 23 (26), 4516-4522 (2004). 2. Yasui, Y. et al., J. Biol. Chem. 279, 12997-13003 (2004).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200123	40002	10 ug	$390.50		Add to Cart

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