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Home / Proteins & Peptide

Aurora Kinase A (Sf9-derived)

SKU : BHP18200125

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Mfr.No.	40004
Immunogen Region	2-403
Tag	N-terminal His-tag
Description	Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 45 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 4 uM Aurora A substrate, and 4 ug/ml Aurora A kinase. Avoid freeze/thaw cycles.
GenBank	NM_003600
Mw(kda)	50 kDa

Synonyms	Aurora Kinase A, STK15, STK6, BTAK, HARK1, ARK1, AURA
Uniprot	O14965

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004). 2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200125	40004	10 ug	$390.50		Add to Cart

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