Human Aurora kinase A also known as, AURKA, Serine/Threonine-Protein Kinase Aurora-A, and Breast Tumor-Amplified Kinase, GenBank Accession No. NM_003600, a.a. 2-403 (end) with N-terminal His-tag, expressed in a HEK 293 cell expression system. MW =46 kDa.
Specific activity: 45 pmole/min/ug.
Immunogen Region
2-403 (end)
Tag
N-terminal His-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200mM Imidazole, 20% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in Kinase buffer containing 2 mM DTT using KEMPtide substrate (0.2 mg/ml) and 10 uM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.
Avoid multiple freeze/thaw cycles.
GenBank
NM_003600
Mw(kda)
46 kDa
Synonyms
AURKA, Serine/Threonine- Protein Kinase Aurora-A, Breast Tumor-Amplified Kinase
Uniprot
O14965
Shipping Temperature
-80°C (dry ice)
Format
Aqueous solution
Storage
At least 6 months at -80°C.
Reference
1. Ouchi, M. et al., J. Biol Chem. 279 (19),19643-19648 (2004). 2. Kunitoku, N. et al., Dev. Cell 5(6), 853-864 (2003).