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Home / Proteins & Peptide

ATP Citrate Lyase, GST-tag

SKU : BHP18200366

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BPS Bioscience

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Mfr.No.	50255
Description	Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system. Host cell line: Sf9 cells Specific activity: >=440 pmole/min/ug.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 25 ul ACLY reaction is conducted in a buffer containing 40 mM Tris (pH 8.0), 10 mM MgCl₂, 2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA and 6-100 ng human ACLY at 30°C for 60 min. Enzyme activity was assayed using ADP-Gloreagents (Promega) Avoid freeze/thaw cycles.
GenBank	NM_001096
Mw(kda)	147 kDa

Synonyms	ATP citrate lyase, ACLY
Uniprot	P53396

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008). 2. Ramakrishna S. et al., Biochemistry 29 (33), 7617-7624 (1990). Application Reference: ATP citrate lyase mediates resistance of colorectal cancer cells to SN38 (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200366	50255	10 ug	$594		Add to Cart

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