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Home / Proteins & Peptide

Ataxin3 (MJD1, SCA3), His-tag

SKU : BHP18200828

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Mfr.No.	80399
Immunogen Region	2-362(end)
Tag	N-terminal His-tag
Description	Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS), Machado-Joseph disease protein 1 (MJD1) or Spinocerebellar ataxia type 3 (SCA3), GenBank Accession No. NM_004993, a.a. 2-362(end) with an N-terminal His-tag, MW=42.1 kDa, expressed in E. coli.
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and Ataxin-3 (0-0.4 ng/uL) in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm. Avoid freeze/thaw cycles.
GenBank	NM_004993
Mw(kda)	42 kDa

Synonyms	Ataxin-3, ATX3, ATXN3, Josephin, JOS, Machado-Joseph disease protein 1, MJD1, Spinocerebellar ataxia type 3, SCA3
Uniprot	P54252

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. do Carmo Costa, M., et al. (2010). PLoS One 5(7):e11728. 2. Jung, J., et al. (2009). Hum Mol Genet. 18(24):4843-52. 3. Macedo-Ribeiro, S., et al. (2009). PLoS One 4(6): e5834.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200828	80399	250 ug	$319		Add to Cart

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