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Home / Proteins & Peptide

ARG1, His-Tag

SKU : BHP18200679

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Mfr.No.	71658
Description	Human Arginase 1 (ARG1) protein, also known as LCN7 and LIECF3, GenBank Accession No. NM_000045, amino acids 1-322, with a C-terminal His-tag, expressed in an HEK293 cell expression system. MW = 36 kDa.
Immunogen Region	1-322
Tag	C terminal His-Tag
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The assay was performed by UV absorption using recombinant ARG1 with thioarginine substrate (Cayman 80240). The UV absorption signal at 412 nm is proportional to the amount of reaction product produced. Reactions were conducted for 30 min at room temperature. To initiate the reaction various concentrations of ARG1 (0-100 nM final concentration) were added to ARG1 assay buffer containing the thioarginine substrate. Absorption signals were measured using a Tecan Infinite M1000 plate reader. Avoid freeze/thaw cycles.
GenBank	NM_000045
Mw(kda)	36 kDa

Synonyms	Liver-Type Arginase 1
Uniprot	P05089

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Sedbrook, J.C., et al., PNAS. 1999 Feb; 96(3):1140-1145.2. Woll, P.J., et al., PNAS. 1998 Mar; 85(6):1859-1863.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200679	71658	50 ug	$544.50		Add to Cart

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