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Home / Proteins & Peptide

aRAF, His-tag

SKU : BHP18200131

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BPS Bioscience

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Mfr.No.	40010
Immunogen Region	282-606 (end)
Tag	N-terminal GST-tag, C-terminal His-tag
Description	Human v-raf murine sarcoma 3611 viral oncogene homolog, transcript variant 1 (A-RAF1), (GenBank Accession No. NM_001654), a.a. 282-606 (end), containing mutations YY301-302DD, with N-terminal GST-tag and C-terminal His-tag, MW=63 kDa, expressed in an Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 40 pmol/min/ug.
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl₂) containing 5 ug inactive MEK1 (BPS #40075), 1 uM ATP, and 4 - 8 ng/ul aRAF for 30 minutes at 30°C. The amount of ATP transferred was measured using a Kinase-Glo Plus Assay Kit (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_001654
Mw(kda)	63 kDa

Synonyms	ARAF1, RAFA1, aRAF
Uniprot	P10398

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yokoyama,T. et al. J. Cell Biol. 177 (5), 781- 793 (2007). 2. Ewing,R.M. et al. Mol. Syst. Biol. 3, 89 (2007).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200131	40010	10 ug	$423.50		Add to Cart

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