Human angiopoietin-like 3(ANGPTL3) also known as angiopoietin-relatedprotein 3, or angiopoietin 5 (Ang5),GenBank Accession No. NM_014495.3,a.a. 1-460 with a C-terminal FLAG tag,expressed in a HEK293 cell expressionsystem.
host species: human
Host species: human
Host cell line: HEK293
Immunogen Region
1-460
Tag
C-terminal FLAG-tag
Formulation
Lyophilized powder from 8mM phosphate, pH 7.4, 100 mM NaCl, and2.2 mM KCl.
Species
Human
Application
Useful for in vitro cell culture assays or in vivo applications.
Endotoxin Level
<1 EU/ug
Biological Activity Comment
Angiopoietin-like 3 activity was confirmed by treating THP-1 cells with 500 ng/ml of angiopoietin-like 3 for 15 min, 30 min, 2 hr, or 4 hr. Phosphorylated-Erk was measured by Western blot and compared to total Erk levels.
GenBank
NM_014495.3
Mw(kda)
52.8 kDa
Synonyms
ANGPTL3, angiopoietin-related protein 3, angiopoietin 5, Ang5
Uniprot
Q9Y5C1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Purity
>=90% as determined by SDS-PAGE
Storage
>12 months at -80°C
Reference
1. Ando, Y., et al., J. Lipid Res. 44: 1216-1223. 2. Lee, E-Chiang, et al., J. Biol. Chem. 284:13735-13745.