JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

AMPK (A1/B1/G1), His-tags

SKU : BHP18200140

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40025
Description	Recombinant full-lengthhuman AMPK (combination of A1/B1/G1subunits) was expressed by baculovirus inSf9 insect cells using a C-terminal His tag.The gene accession number for the threesubunits (A1/B1/G1) is NM_006251,NM_006253, and NM_002733,respectively. This protein has beenactivated with CAMKK. host species: Sf9 cells Host species: Sf9 cells Specific activity: >=700 pmole/min/ug
Background	AMPK is a heterotrimer protein kinase consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Immunogen Region	full length
Tag	C-terminal His-tags
Formulation	50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 1 mM DTT using AMARApeptide as a substrate (0.1 mg/ml), 20 mM ATP and 100 uM AMP . Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_006251(A1), NM_006253(B1), NM_002733 (G1)
Mw(kda)	~146 kDa (complex)

Synonyms	Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666
Uniprot	A1: Q13131; B1: Q9Y478; G1: P54619

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=95%
Storage	At least 6 months at -80°C.

Reference	1. Minokoshi, Y. et al. Nature 428: 569-574, 2004. 2. Hardie, D G. et al. Eur J Biochem. 1997 Jun 1,246(2):259-73.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200140	40025	10 ug	$676.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.