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Home / Proteins & Peptide

AKT3, His-tag

SKU : BHP18200133

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BPS Bioscience

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Mfr.No.	40012
Description	Recombinant human AKT3 (GenBank Accession No. NM_005465), a.a. 2-end, with N-terminal His-tag, MW= 60 kDa, expressed in a baculovirus expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 14 pmol/min/ug
Immunogen Region	2-end
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_005465
Mw(kda)	60 kDa

Synonyms	AKT3, PKBgamma, PKBgamma, RACgamma, AKT
Uniprot	Q9Y243

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Coffer, PJ. et al. Biochem J. 1998 Oct 1; 335 (Pt 1):1-13. 2. Anderson, KE. et al. Curr Biol. 1998 Jun 4;8(12): 684-91.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200133	40012	10 ug	$390.50		Add to Cart

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