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Home / Proteins & Peptide

AKT2, Active, His-tag

SKU : BHP18200132

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BPS Bioscience

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Mfr.No.	40011
Description	Recombinant human AKT2 (GenBank Accession No. NM_001626), full length with N-terminal His-tag, MW=60.2 kDa, was expressed in Sf9 insect cells via a baculovirus expression system. The purified AKT2 is activated in vitro using PDK1 enzyme. host species: Sf9 cells Host species: Sf9 cells Specific activity: 7 pmol/min/ug
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Purification	Purified by affinity chromatography
Notes	The enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 6, Invitrogen) at 30 °C for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_001626
Mw(kda)	57 kDa

Synonyms	AKT2, PKBbeta, PKBbeta, RACbeta, AKT
Uniprot	P31751

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Alessi et al., 1998. Current Biology 8: 69. 2. Cohen et al., 1997. FEBS Lett. 410: 3.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200132	40011	10 ug	$390.50		Add to Cart

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