Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: Inactive
Immunogen Region
1-480(end)
Tag
N-terminal His-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.2mM KCl, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful as a substrate in kinase assays.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_001014432
Mw(kda)
60.2 kDa
Synonyms
AKT1, PKBalpha, PKBalpha, RACalpha, AKT
Uniprot
P31749
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Alessi et al., Current Biology 8: 69, 1998. 2. Cohen et al., FEBS Lett. 410: 3, 1997.Application Reference:Arachidonoyl-phosphatidylcholine oscillates during the cell cycle and counteracts proliferation by suppressing Akt membrane binding (2013)