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AKT1, Active

SKU : BHP18200124

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BPS Bioscience

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Mfr.No.	40003
Description	Human AKT Serine/Threonine Kinase 1 (AKT1), also known as Protein Kinase B Alpha (PKBalpha), and RAC, GenBank Accession No. NM_001014432, a.a. 1-480(full length) with N-terminal His-tag, was expressed in Sf9 insect cells via a baculovirus expression system. MW=60 kDa. The purified AKT1 is activated in vitro using PDK1 enzyme. host species: Sf9 cells Host species: Sf9 cells Specific activity: >100 pmol/min/ug
Immunogen Region	1-480(full length)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM Imidazole and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Purification	Purified by affinity chromatography
Notes	Assay was done in Kinase buffer containing 1 mM DTT using Crosstide substrate (0.1 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_001014432
Mw(kda)	60 kDa

Synonyms	AKT1, PKBalpha, PKBalpha, RACalpha, AKT
Uniprot	P31749

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kuijl, C., et al., Nature. 2007 Nov 29; 450(7170):725-30. 2. Vogiatzi, P, and Giordano, A., Cancer Biol Ther. 2007 Aug 3; 6(10).Product Specific Reference(s): 1. Koeberle, A., et al., Proc Natl Acad Sci U S A. 2013 Feb 12; 110(7):2546-51.Application ReferencesPhosphorylation-Dependent Differences in CXCR4-LASP1-AKT1 Interaction between Breast Cancer and Chronic Myeloid Leukemia

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200124	40003	10 ug	$412.50		Add to Cart

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