HSC70 Protein - BHP11900016

Mfr.No.	SPR-106, SPR-106A, SPR-106B, SPR-106C
Description	Active Human Recombinant HSC70 Protein
Alternative Names	Constitutive heat shock protein 70 Protein, HSC54 Protein, HSC71 Protein, HSC73 Protein, HSP71 Protein, HSP74 Protein, HSPA10 Protein, HSPA8 Protein, LAP1 Protein, NIP71 Protein
Biological Activity	ATPase active
Cellular Localization	Cytoplasm, Melanosome
Certificate Of Analysis	This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2uM phosphate liberated/hr/ug protein in a 200ul reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Regulatory	RUO
Scientific Background	HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
Species	Human
Specificity	~70 kDa
Accession Number	AF352832
Concentration	Lot/batch specific. See included datasheet.
Purification	Affinity Purified
Expression System	E. coli
Bioactivity	ATPase active
Biological Activity Abreviation	Active
Biological Activity Comment	ATPase active
Cite This Product	Human Recombinant HSC70 Protein (StressMarq Biosciences, Canada, Cat # SPR-106A)
Mol Weight	~70 kDa
Nature	Recombinant

Gross Weight (kg)	1.4
Launch Date	2007/09/01
Net Weight (g)	0.05
Number Of Pubmed Citations	8
Swissprot	P11142
Target Details	HSC70
Gene Id	3312
Research Area	Cancer, Heat Shock
Target	HSC70

Adr Code	Non-hazardous
Country Of Origin	Canada
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Published Application	Functional Assay ; Western Blot ; Functional Assay ; Intrinsic fluorescence spectroscopy ; Western Blot Control ; Functional Assay ; Functional Assay ; Western Blot Control
Published Species Reactivity	Rat ; Human ; Human ; Mouse ; Photinus pyralis ;
Pubmed Id	28110910 ; 28026090 ; 26244546 ; 25939977 ; 25979775 ; 23291500 ; 20084527 ; 19209902
Shipping	Blue Ice or 4ºC
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Blue Ice or 4°C
Buffer	50mM Tris/HCl, pH8, 0.3M NaCl
Description	Accession Number: AF352832 Specificity: ~70 kDa Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay. Country of Origin: Canada Gross Weight (kg): 1.4 Net Weight (g): 0.05 Stock Product: Y
Purity	>90%
Storage Buffer	50mM Tris/HCl, pH8, 0.3M NaCl
Storage Temperature	-20°C

Immunogen Species	Homo Sapiens (Human)
Applications	ATPase Activity assay, Functional assay, WB, ELISA, SDS-PAGE

Reference	1. Brown C. L., et al. (1993) J.Cell Biol. 120(5): 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271(2): 833-840.

Product Manual	Datasheet
SDS

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Combination SKU	Supplier No.	Size	Your price
BHP11900016-1	SPR-106A	50 ug	$197	Add to Cart
BHP11900016-2	SPR-106B	100 ug	$281	Add to Cart
BHP11900016-3	SPR-106C	100 ug x2	$542	Add to Cart

Active Human Recombinant HSC70 Protein

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