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Home / Proteins & Peptide

Activated Protein C (PROC), His-tag

SKU : BHP18200551

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Mfr.No.	70019
Description	Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions. Specific activity: >= 95 pmol/min/ug
Immunogen Region	1-461 (end)
Tag	C-terminal His-tag
Formulation	50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl₂, 0.01% Brij-35, 100 uM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460. Avoid freeze/thaw cycles.
GenBank	NM_000312

Synonyms	PROC, APC, activated protein C, active coagulation factor XIV
Uniprot	P04070

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340. 2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200551	70019	20 ug	$484		Add to Cart

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