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Home / Proteins & Peptide

ACK1, GST-tag

SKU : BHP18200232

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BPS Bioscience

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Mfr.No.	40400
Description	Human Ack1 (GenBank Accession No. NM_005781), a.a. 110-476(end) with N-terminal GST-tag, MW=68 kDa, expressed in Sf9 insect cells via abaculovirus expression system. Host cell line: Sf9 cells
Immunogen Region	110-476(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 minutes. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_005781
Mw(kda)	68 kDa

Synonyms	ACK1, activated Cdc42-associated kinase 1
Uniprot	Q07912

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Eley, L. et al., Biochem. Biophys. Res. Commun. 371 (4), 877-882 (2008). 2. Grovdal, L.M. et al., Exp. Cell Res. 314 (6), 1292-1300 (2008).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200232	40400	10 ug	$390.50		Add to Cart

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