Human Angiotensin converting enzyme 2 (ACE2) also known ACEH, GenBank Accession No. NP_068576.1, a.a. 18-740, with a C-terminal His-tag, expressed in a HEK293 expression system. MW=85 kDa.
Immunogen Region
18-740
Tag
C-terminal His-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCL, 2.2mM KCl, 200 mM Imidazole and 20% glycerol.
Species
Human
Application
As an exopeptidase, ACE2 is useful for the study of enzyme kinetics and to screen for inhibitors. As a cell receptor for coronaviruses infection, including 2019-nCoV, ACE2 is useful for the study of protein-protein interactions.
Notes
ACE2 exopeptidase activity was measured in a 50 ul reaction containing L-Phe quantification reagents in 50 mM Tris pH 8.0, as previously described. Fluorescence intensity was kinetically recorded at 555 nm excitation/585 nm emission, and net initial rates, measured in RFU/min, were used to plot the results.