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Home / Proteins & Peptide

ACC2, His-tag

SKU : BHP18200362

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BPS Bioscience

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Mfr.No.	50201
Description	Human acetyl-Coenzyme A carboxylase alpha transcript variant 2 (ACC2), GenBank Accession No. NM_001093, full length, with C-terminal His-tag, MW=277 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: >=41 pmoles/min/ug
Immunogen Region	full length
Tag	C-terminal His-tag
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 25 ul ACC2 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl₂, 2 mM potassium citrate, 1 mM DTT, 100 uM ATP, 12 mM NaHCO₃ and 20 uM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo Luminescent Assay Platform (Promega). Avoid freeze/thaw cycles.
GenBank	NM_001093
Mw(kda)	277 kDa

Synonyms	acetyl-Coenzyme A carboxylase alpha transcript variant 2, ACC-2, ACC 2, ACC -2
Uniprot	O00763

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Locke G.A. et al., Arch. Biochem. Biophys. 475 (1), 72-79 (2008). 2. Abu-Elheiga L. et al., Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1444-1449 (2000).Application Reference(s): A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200362	50201	10 ug	$638		Add to Cart

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