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Home / Proteins & Peptide

ACC1, FLAG-His-tags

SKU : BHP18200363

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BPS Bioscience

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Mfr.No.	50202
Description	Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1-2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: >=35 pmol/min/ug.
Immunogen Region	1-2383(end)
Tag	C-terminal FLAG-His-tags
Formulation	50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 1 mM DTT, 100 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 25 ul ACC1 reaction is conducted in abuffer containing 30 mM HEPES (pH 7.4), 2mM MgCl₂, 2 mM potassium citrate, 1 mMDTT, 100 uM ATP, 12 mM NaHCO₃ and 20 uM acetyl-CoA at 30°C for 45 min. ATPreduction is detected using ADP-Glo Luminescent Assay Platform (Promega). Avoid freeze/thaw cycles.
GenBank	NM_198834
Mw(kda)	271 kDa

Synonyms	acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1
Uniprot	Q13085

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007) 2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006)Application Reference(s): A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200363	50202	10 ug	$638		Add to Cart

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