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Home / Proteins & Peptide

Abl Kinase Mutant (T315I), His-tag

SKU : BHP18200239

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BPS Bioscience

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Mfr.No.	40415
Description	Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1), GenBank Accession No. NM_005157, a.a. 229-512, with Thr-to-Ile mutation on a.a. 315* and N-terminal His-tag, MW = 33 kDa, expressed in a baculovirus-infected Sf9 cell expression system. *This mutation is often cited as T344I in variant B (GenBank Accession No. NM_007313). Host cell line: Sf9 cells Specific activity: 222 pmoles/min/ug.
Immunogen Region	229-512
Tag	N-terminal His-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Activity was determined using a 100% phosphorylated Tyr02 peptide and a Z-lyte kinase assay (Invitrogen). Reaction was incubated at 30°C for 1 hour. This mutation is also cited as T344I in variant B (GenBank Accession No. NM_007313). Avoid freeze/thaw cycles.
GenBank	NM_00731
Mw(kda)	33 kDa

Synonyms	Abl-1(T315I), Abl-1(T344I), ABL1, Abelson murine leukemia viral oncogene homolog 1, JTK7
Uniprot	P00519

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Cao, C., et al. 2003. J. Biol. Chem. 278(15): 12961-7. 2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3): 356-364.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200239	40415	10 ug	$390.50		Add to Cart

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