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Home / Proteins & Peptide

Abl Kinase, His-tag

SKU : BHP18200237

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BPS Bioscience

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Mfr.No.	40411
Description	Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 34.8 kDa, expressed in a Baculovirus-infected Sf9 cell expression system Host cell line: Sf9 cells Specific activity: 2,700 pmoles/min/ug.
Immunogen Region	248-531
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_00731
Mw(kda)	34.8 kDa

Synonyms	ABL1, ABL-1, Abelson murine leukemia viral oncogene homolog 1, JTK7
Uniprot	P00519

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961-7. 2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356-364.Application Reference:LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia (2014)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200237	40411	10 ug	$412.50		Add to Cart

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