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Home / Proteins & Peptide

A20, His tag, FLAG-tag (Sf9-derived)

SKU : BHP18200836

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BPS Bioscience

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Mfr.No.	80408
Description	Human A20, also known astumor necrosis factor- induced protein 3(TNFAIP3) or OTU domain-containingprotein 7C (OTUD7C), GenBank AccessionNo. NM_006290, amino-acids 2-790(end)with an N-terminal FLAG-tag and C-terminalHis-tag, MW=91 kDa, expressed in Sf9insect cells via a baculovirus expressionsystem. Specific activity: 0.766 pmol/min/ug
Immunogen Region	2-790(end)
Tag	N-terminal FLAG-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performedin 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mMEDTA, 500 nM Ub-AMC, and variousamount of A20 in a volume of 50 uL.Reaction was incubated at 37°C for 30 minand fluorescent signal was measured atexcitation of 360 nm, and emission at 460nm. Avoid freeze/thaw cycles.
GenBank	NM_006290
Mw(kda)	91 kDa

Synonyms	tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C, A20
Uniprot	Q9UHC1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7. 2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9. 3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200836	80408	50 ug	$484		Add to Cart

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