Recombinant SARS-CoV-2 3C-like Protease, full length, encompassing amino acids 1-306(end). This construct contains an N-terminal His-tag followed by a C-terminal GST-Tag. The recombinant protein contains mutations of interests (T21I) and (A173V) and is affinity purified. Constructs: 3CL Protease (T21I, A173V) (GST-1-306(end)-His) (SARS-CoV-2),mutation=T21I, A173V
host species: E. coli
Host species: E. coli
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Mutation
T21I, A173V
Species
SARS-CoV-2
Notes
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles. Avoid freeze/thaw cycles