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Home / Proteins & Peptide

3CL Protease, MBP-tag, His-tag (MERS-CoV)

SKU : BHP18201274

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BPS Bioscience

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Mfr.No.	100757-1
Description	Middle East Respiratory Syndrome Coronavirus 3C-like protease (MERS-CoV 3CL Protease), GenBank Accession No. ATG84821.1, a.a. 1-306(full length), with an N-terminal MBP-tag, and C-terminal His-tag, expressed in an E. coli expression system, MW=78 kDa.
Immunogen Region	1-306(full length)
Tag	N-terminal MBP-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 20% glycerol, variable Imidazole.
Species	MERS-CoV
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Add 40 ul of MERS-CoV 3CL protease diluted in 1x assay buffer. Start the reaction by adding 10 ul of diluted substrate. Incubate 4 hours at 37°C. Read fluorescence (lambdaexc=485 nm, lambdaem=528 nm). Total volume is 50 ul. Avoid freeze/thaw cycles.
GenBank	ATG84821.1
Mw(kda)	78 kDa

Synonyms	coronavirus, 3C-like protease, MERS protease, MERS-ncov protease, covid, covid-19, covid19, MERS-cov, MERS cov, merscov2, Mpro, Main protease,100757-1, 100757-2
Uniprot	A0A291I686

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Jo, S. et al., J. Enzyme Inh. & Med. Chem. 2019; 1(35): 145-151. 2. He, J. et al., Intl J. Antimicrobial Agents. 2020; doi.org/10.1016/j.ijantimicag.2020.106055.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201274	100757-1	100 ug	$379.50		Add to Cart

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