Purified recombinant SARS-CoV-2 protease 3CL (3C-like protease, also known as MPro) full length encompassing amino acids 1-306 (end). This construct does not contain a tag. The protein corresponds to SARS-CoV-2 variant B.1.1.529 (Omicron Variant), originally discovered in South Africa, and contains mutation P132H. Constructs: 3CL Protease (B.1.1.529, Omicron Variant) (1-306(end))(P132H)(SARS-CoV-2)
Immunogen Region
1-306(end)
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol, and 3 mM DTT.
Methods
Fluorogenic
Mutation
P132H
Species
SARS-CoV-2
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done according to the 3CL Protease, MBP-tagged (SARS-CoV-2) Assay Kit (Biohippo #79955-1) with 3CL Protease (B.1.1.529, Omicron Variant) titrated from 90 ng/rxn to 0 ng/rxn.